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Observed that though RTG20-D is homozygous for his4::LEU2 allele, and hence histidine auxotroph, it carries heterozygous genotype at SNP position within the vicinity of HIS4 locus, suggesting that RTG20-D histidine auxotrophy resulted from a NCO occasion involving the HIS4 locus. Thus, we investigated if exclusion of the RTG20-D strain in the evaluation improved the mapping. It did not, rather su
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