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N transporter CglA while ccs4 and ccs16 are competence-induced proteins. Competence regulation was not the primary target of DM3 in standalone or in combination with PEN. Of note, CoiA, which functions to promote genetic recombination during transformation45, was only found in cells treated with DM3PEN suggesting the unique effect of the synergistic treatment in enhancing transformational recombi
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On electron micrograph reported extensive cell wall and cell membrane lysis processes in DM3-treated pneumococcal cells. Together with the absence of differential autolysin expression in DM3-treated group in this study, it is suggested that other lytic mechanisms could be involved. In addition, membrane and cell wall associated structural components and transport mechanisms were greatly affected

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